anti h3k9me2 3 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti h3k9me2 3
Anti H3k9me2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/histone+h3/Di%2FTri-Methyl-Histone+H3+(Lys9)+Mouse+mAb/pmc12989073-223-44-47
Average 94 stars, based on 83 article reviews
Anti H3k9me2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/histone+h3/Di%2FTri-Methyl-Histone+H3+(Lys9)+Mouse+mAb/pmc12989073-223-44-47
Average 94 stars, based on 83 article reviews
anti h3k9me2 3 - by Bioz Stars,
2026-09
94/100 stars
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Immunoprecipitation:Article Title: HIF-1 A-Mediated Lactate Metabolism Confers Ferroptosis Resistance in M1 Macrophages Through Histone Lactylation During Acute Lung Injury. Article Snippet: .. Immunoprecipitation was performed overnight at 4°C using 5 μg of antibodies: Negative Control:Article Title: HIF-1 A-Mediated Lactate Metabolism Confers Ferroptosis Resistance in M1 Macrophages Through Histone Lactylation During Acute Lung Injury. Article Snippet: .. Immunoprecipitation was performed overnight at 4°C using 5 μg of antibodies: Blocking Assay:Article Title: SAA/FPR2 Signaling Between Pericentral Hepatocytes and Macrophages Exacerbates Zonated Liver Transplant Injury. Article Snippet: Then, protein samples were separated by 12% SDS-PAGE and subsequently transferred onto 0.45 μm PVDF membranes (Merck Millipore, USA). .. After blocking with 5% skim milk for 1 h at RT, the membranes were incubated overnight at 4◦C with primary antibodies against: FOXO1 (1:1000; Cell Signaling Technology, USA), SAA (1:1000; Proteintech, China), Incubation:Article Title: SAA/FPR2 Signaling Between Pericentral Hepatocytes and Macrophages Exacerbates Zonated Liver Transplant Injury. Article Snippet: Then, protein samples were separated by 12% SDS-PAGE and subsequently transferred onto 0.45 μm PVDF membranes (Merck Millipore, USA). .. After blocking with 5% skim milk for 1 h at RT, the membranes were incubated overnight at 4◦C with primary antibodies against: FOXO1 (1:1000; Cell Signaling Technology, USA), SAA (1:1000; Proteintech, China), Article Title: Rpl12 paralog dependent TOR-signaling controls the expression of ribosome preservation factor Stm1 Article Snippet: Total cell lysates and ribosome fractions were separated by SDS-PAGE and the proteins were transferred on to nitrocellulose membrane (0.45 μm pore size; BioRad). .. Membranes were blocked with 5% BSA for 1 hour at room temperature incubated overnight at 4 °C with primary antibodies (1:2000 dilution) against Rpl12 (Invitrogen), phospho-Rps6 (Ser235/236), Rps6, and Article Title: MK2/p38/p53 Suppress Basal IL-1β and Non-Canonical NF-κB Signaling in Macrophages Article Snippet: An amount of 20–40 μg of protein was mixed with 4× Laemmli’s SDS sample buffer, heated at 95 ◦C for 5 min, separated by SDS-PAGE on 7.5% to 16% gradient gels with a PageRuler Prestained Protein Ladder (26616, Thermo Fisher Scientific Inc., Waltham, MA, USA) and transferred to Hybond ECL nitrocellulose membranes (GE Healthcare, Düsseldorf, Germany) through semidry blotting. .. The primary antibodies GAPDH (MAB374, Merck Millipore, Merck KGaA, Darmstadt, Germany), IκBa (9242, Cell Signaling Technology (CST, Cambridge, UK), MDM2 (D-7) (sc-13161, SCBT, Dallas, TX, USA), EF2 (C-9) (sc166415, SCBT, Dallas, TX, USA), NF-κB1 p105/p50 (D7H5M) (12540, CST, Cambridge, UK), https://doi.org/10.3390/ijms27073232 NF-κB2 p100/p52 (4882, CST, Cambridge, UK), NF-κB p65 (L8F6) (6956, CST, Cambridge, UK), RelB (C1E4) (4922, CST, Cambridge, UK), p53 (1C12) (2524, CST, Cambridge, UK), cRel (D4Y6M) (12707, CST, Cambridge, UK), Mouse IL-1β/ IL-1F2 (AF 401 SP, R&D systems, Bio-Techne GmbH, Wiesbaden-Nordenstadt, Germany), Control:Article Title: Selenoprotein P deficiency drives hepatocellular carcinoma progression via induction of neutrophil senescence and immunosuppressive microenvironment. Article Snippet: .. The primary antibodies used in the experiments were antibodies with specificity for SEPP1 (1:300, Santa Cruz, Cat. sc- 376858), |
![( A ) Metabolism pathway enrichment analysis of DEGs in DAM2 microglial cluster. ( B ) Lipid/cholesterol metabolism–related DEGs in DAM2. mTOR, mechanistic target of rapamycin. ( C ) Retinal section stained with Filipin (free cholesterol; magenta), Iba-1 (microglia; green), and <t>Histone</t> <t>H3</t> (nuclei; white) antibodies and quantification of Filipin + microglia in the ONL of the retinas from rd10 retinas administrated with AAV- Cx3cr1-Tpm1 -EGFP or control vector ( n = 5 mice per group). Yellow arrowheads indicate Filipin + microglia. Scale bar, 20 μm. For image acquiring, three views in each retinal section at 100 μm (central), 1 mm (middle), and 1.8 mm (peripheral) from the optic nerve head along the dorsal and ventral directions were captured. ( D ) qPCR validation of Tpm1 and lipid metabolism regulators ( Apoe , Fabp5 , Apoc1 , Plin2 , Lypla1 , Sp1 , and Soat1 ) in primary microglia transfected with Tpm1 plasmid versus control ( n = 3 independent experiments). ( E to G ) Fluorescent colocalization analysis of lipid and cholesterol accumulation (Nile Red: lipid droplets; BODIPY C11: neutral lipids; Filipin: free cholesterol) in microglia following Tpm1 overexpression and Apoe/Fabp5 knockdown. Cell percentages of Nile Red + , BODIPY C11 + , or Filipin + microglia were quantified ( n = 3 independent experiments). Scale bars, 20 μm. ( H ) Transcriptional profiling of inflammatory mediators in dual-modified microglia ( n = 3 independent experiments). ( I ) Schematic model: Tpm1 up-regulation drives Apoe/Fabp5 -dependent lipid accumulation in microglia, promoting neuroinflammatory responses and photoreceptor degeneration in RP. Data represent means ± SEMs. Statistical significance determined using unpaired two-tailed Student’s t test (C) or one-way ANOVA with Tukey’s multiple-comparison test [(D) to (H)] (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5182/pmc13215182/pmc13215182__sciadv.aea6467-f7.jpg)