anti h3k9me2 3 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti h3k9me2 3
Anti H3k9me2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/histone+h3/Di%2FTri-Methyl-Histone+H3+(Lys9)+Mouse+mAb/pmc12989073-223-44-47
Average 94 stars, based on 83 article reviews
Anti H3k9me2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/histone+h3/Di%2FTri-Methyl-Histone+H3+(Lys9)+Mouse+mAb/pmc12989073-223-44-47
Average 94 stars, based on 83 article reviews
anti h3k9me2 3 - by Bioz Stars,
2026-10
94/100 stars
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Immunoprecipitation:Article Title: HIF-1 A-Mediated Lactate Metabolism Confers Ferroptosis Resistance in M1 Macrophages Through Histone Lactylation During Acute Lung Injury. Article Snippet: .. Immunoprecipitation was performed overnight at 4°C using 5 μg of antibodies: Negative Control:Article Title: HIF-1 A-Mediated Lactate Metabolism Confers Ferroptosis Resistance in M1 Macrophages Through Histone Lactylation During Acute Lung Injury. Article Snippet: .. Immunoprecipitation was performed overnight at 4°C using 5 μg of antibodies: Blocking Assay:Article Title: SAA/FPR2 Signaling Between Pericentral Hepatocytes and Macrophages Exacerbates Zonated Liver Transplant Injury. Article Snippet: Then, protein samples were separated by 12% SDS-PAGE and subsequently transferred onto 0.45 μm PVDF membranes (Merck Millipore, USA). .. After blocking with 5% skim milk for 1 h at RT, the membranes were incubated overnight at 4◦C with primary antibodies against: FOXO1 (1:1000; Cell Signaling Technology, USA), SAA (1:1000; Proteintech, China), Incubation:Article Title: SAA/FPR2 Signaling Between Pericentral Hepatocytes and Macrophages Exacerbates Zonated Liver Transplant Injury. Article Snippet: Then, protein samples were separated by 12% SDS-PAGE and subsequently transferred onto 0.45 μm PVDF membranes (Merck Millipore, USA). .. After blocking with 5% skim milk for 1 h at RT, the membranes were incubated overnight at 4◦C with primary antibodies against: FOXO1 (1:1000; Cell Signaling Technology, USA), SAA (1:1000; Proteintech, China), Article Title: Rpl12 paralog dependent TOR-signaling controls the expression of ribosome preservation factor Stm1 Article Snippet: Total cell lysates and ribosome fractions were separated by SDS-PAGE and the proteins were transferred on to nitrocellulose membrane (0.45 μm pore size; BioRad). .. Membranes were blocked with 5% BSA for 1 hour at room temperature incubated overnight at 4 °C with primary antibodies (1:2000 dilution) against Rpl12 (Invitrogen), phospho-Rps6 (Ser235/236), Rps6, and Control:Article Title: Selenoprotein P deficiency drives hepatocellular carcinoma progression via induction of neutrophil senescence and immunosuppressive microenvironment. Article Snippet: .. The primary antibodies used in the experiments were antibodies with specificity for SEPP1 (1:300, Santa Cruz, Cat. sc- 376858), other:Article Title: MK2/p38/p53 Suppress Basal IL-1β and Non-Canonical NF-κB Signaling in Macrophages Article Snippet: RNA was extracted using TRIzol Reagent (Invitrogen, Thermo Fisher Scientific Inc., Waltham, MA, USA), and 0.5μg RNA (according to the NanoDrop ND-1000 spectrophotometer, Peqlab Biotechnologie GmbH, Erlangen, Germany) was converted to cDNA through reverse transcriptase PCR with Biometra TRIO (Analytik Jena GmbH+Co. |
![( A ) Metabolism pathway enrichment analysis of DEGs in DAM2 microglial cluster. ( B ) Lipid/cholesterol metabolism–related DEGs in DAM2. mTOR, mechanistic target of rapamycin. ( C ) Retinal section stained with Filipin (free cholesterol; magenta), Iba-1 (microglia; green), and <t>Histone</t> <t>H3</t> (nuclei; white) antibodies and quantification of Filipin + microglia in the ONL of the retinas from rd10 retinas administrated with AAV- Cx3cr1-Tpm1 -EGFP or control vector ( n = 5 mice per group). Yellow arrowheads indicate Filipin + microglia. Scale bar, 20 μm. For image acquiring, three views in each retinal section at 100 μm (central), 1 mm (middle), and 1.8 mm (peripheral) from the optic nerve head along the dorsal and ventral directions were captured. ( D ) qPCR validation of Tpm1 and lipid metabolism regulators ( Apoe , Fabp5 , Apoc1 , Plin2 , Lypla1 , Sp1 , and Soat1 ) in primary microglia transfected with Tpm1 plasmid versus control ( n = 3 independent experiments). ( E to G ) Fluorescent colocalization analysis of lipid and cholesterol accumulation (Nile Red: lipid droplets; BODIPY C11: neutral lipids; Filipin: free cholesterol) in microglia following Tpm1 overexpression and Apoe/Fabp5 knockdown. Cell percentages of Nile Red + , BODIPY C11 + , or Filipin + microglia were quantified ( n = 3 independent experiments). Scale bars, 20 μm. ( H ) Transcriptional profiling of inflammatory mediators in dual-modified microglia ( n = 3 independent experiments). ( I ) Schematic model: Tpm1 up-regulation drives Apoe/Fabp5 -dependent lipid accumulation in microglia, promoting neuroinflammatory responses and photoreceptor degeneration in RP. Data represent means ± SEMs. Statistical significance determined using unpaired two-tailed Student’s t test (C) or one-way ANOVA with Tukey’s multiple-comparison test [(D) to (H)] (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5182/pmc13215182/pmc13215182__sciadv.aea6467-f7.jpg)